Aims: This study was undertaken to analyze total phenolics and total flavonoids contents;\r\nand total antioxidant capacity of pomegranate peel extract and to identify the major\r\nfunctional components in the extract.\r\nStudy Design: The extract was subjected to ESI-MS/MS.\r\nPlace and Duration of Study: Department of Food Science and Nutrition, Sultan Qaboos\r\nUniversity and DARIS Research Center, University of Nizwa, between December 2011\r\nand August 2012.\r\nMethodology: Pomegranate peel extract was analyzed using a Waters Quattro Premier\r\nXE tandem quadrupole mass spectrometer (Waters Corporation, Manchester, UK)equipped with electro-spray ionization (ESI) source. Instrument control and data\r\nacquisition were performed using Mass Lynx ver. 4.1 software. The instrument was\r\ncalibrated for nominal resolution for MS1 and MS2 up to 1200 m/z using the sodium\r\ncaesium iodide standard calibration solution.\r\nResults: Results revealed high contents of total phenolics (64.2 mg Gallic acid\r\nequivalent/ g dry solids) and total flavonoids (1.4 mg Catechin equivalent/ g dry solids)\r\nrespectively. Total antioxidant capacity ranged from 42.3 ââ?¬â?? 461.2 Ã?µmolTrolox equivalent/ g\r\ndry solids. The analysis revealed the presence of 61 different polyphenols in the extract\r\namong which 12 hydroxycinnamic acids, 14 hydrolysable tannins, 9 hydroxybenzoic\r\nacids, 5 hydroxybutanedioic acids, 11 hydroxy-cyclohexanecarboxylic acids and 8\r\nhydroxyphenyls. Major compounds were tannins and flavonoids such as; illogic acid,\r\ngallic acids, punicalin, and punicalagin.\r\nConclusion: A wide variety of phytochemicals present in pomegranate peel extract were\r\nidentified. These functional compounds in pomegranate peels could be utilized by the\r\nfood industry and pharma/nutraceuticalââ?¬â?¢s industry. Further work should be done to isolate\r\nand quantify major functional compounds of pomegranate peels such as ellagic acid.
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